Regulatory
L7AeO

Part:BBa_K3179001:Design

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-13)


L7AeO(a optimized L7Ae which is a transcriptional level repressor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a mutation of previously part L7Ae. L7Ae is a switch of L7Ae-Kturn System. In this part, we mutant the 106th A to C and make the T to P after translation, which can lead to function losing of L7Ae because of the braking down of α-helix. In our design, this part is used to prove the suppress efficiency of L7Ae.

Source

We get this sequence from the previous part L7Ae, and the sequence was synthesized from a biological company.

References